Evidence memo / Reproductive & endocrine

Cosyntropin

Also: Cortrosyn · Synacthen · tetracosactide · ACTH 1-24

Cosyntropin (Cortrosyn) is a synthetic fragment of ACTH used mainly as a diagnostic agent. In the ACTH stimulation test, cosyntropin is given and the cortisol response is measured to help assess adrenal gland function. Its diagnostic use is well established. The Watchlist boundary is keeping it to its diagnostic role and not confusing it with therapeutic ACTH products.

Approved for specific usesPost-market evidence

The verdict

Our read at a glance.

Whether cosyntropin's established diagnostic role in testing adrenal function is understood as a diagnostic agent rather than a treatment.

The signal
Decades of clinical use and study support cosyntropin for the ACTH stimulation test to evaluate adrenal insufficiency. It produces a measurable cortisol rise in people with healthy adrenal function.
The unknown
Mostly procedural questions, such as test thresholds and dosing variations, rather than whether the test works.
Our read
A standard diagnostic tool, not a treatment. Cosyntropin is well established for testing adrenal function, so the main point is keeping it in its diagnostic lane and distinct from therapeutic ACTH products.

The mechanism

How it works.

Cosyntropin is a synthetic peptide made of the first 24 amino acids of ACTH, the part of the pituitary hormone that carries its full biological activity. Injected as a test dose, it binds melanocortin-2 receptors on the adrenal cortex and signals it to release cortisol, exactly as natural ACTH does. Measuring how much cortisol rises afterward shows whether the adrenal glands can respond normally, which is what the ACTH stimulation test checks.

Safety & regulatory boundary

The consequential part.

Cosyntropin is generally well tolerated as a diagnostic agent, with hypersensitivity reactions the main concern. It is a synthetic ACTH fragment used for diagnosis and is not the same as therapeutic ACTH gel products or compounded or research-labeled cosyntropin, and its diagnostic use does not extend to treatment or wellness claims.

What's next

What we're watching.

Standardization of test protocols and thresholds, and supply or product-identity issues across cosyntropin products.

Context

Why this is discussed.

Cosyntropin is the standard tool for the ACTH stimulation test, a common way to check whether the adrenal glands respond normally.

The evidence base

23 cited references.

Regulatory & labels3

DailyMed label: Cosyntropin

Medical Purchasing Solutions, LLC (FDA label) · 2023

DailyMed label: Cosyntropin

Sandoz Inc (FDA label) · 2024

DailyMed label: Cortrosyn

Medical Purchasing Solutions, LLC (FDA label) · 2024

Registered trials8

Adrenal Function in Critical Illness

ClinicalTrials.gov · COMPLETED · 2004-11-12

Polycystic Ovary Syndrome in Type 1 Diabetes

ClinicalTrials.gov · NOT_YET_RECRUITING · 2026-04-01

Opioid-Free Anesthesia in Cardiac Surgery

ClinicalTrials.gov · PHASE3 · COMPLETED · 2021-07-22

Updated Diagnostic Cortisol Values for Adrenal Insufficiency

ClinicalTrials.gov · NA · RECRUITING · 2022-02-03

Nebulized Nasal Steroids

ClinicalTrials.gov · PHASE1/PHASE2 · NOT_YET_RECRUITING · 2025-12

Indexed literature (PubMed)12

Adrenal Insufficiency in Adults: A Review

Vaidya A et al. · JAMA · 2025

Congenital adrenal hyperplasia

El-Maouche D et al. · Lancet · 2017

Adrenocortical insufficiency

Burke CW · Clin Endocrinol Metab · 1985

Corticotroph deficiency

Lefebvre H · Ann Endocrinol (Paris) · 2012

Secondary hypoadrenalism

Reimondo G et al. · Pituitary · 2008

Relative adrenal insufficiency

Loriaux DL et al. · Curr Opin Endocrinol Diabetes Obes · 2009

Adrenal venous sampling: cosyntropin stimulation or not?

Deinum J et al. · Eur J Endocrinol · 2019

Keep reading

Related published memos.